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To test how meth concentrations in surface water around the world might affect aquatic species, the Czech researchers put a group of 60 brown trout into a holding tank, then exposed them to one microgram of meth per liter of water — the typical amount of the drug found downstream of Czech and Slovak wastewater plants. They kept the trout in the water for two months, while a control group of another 60 trout swam in a tank without meth.